FastQCFastQC Report
Mon 3 Jul 2023
SRR957824_50K_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR957824_50K_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000
Total Bases7.5 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGGAGAGGGGCGGGGGGGGGAAGAGGGGAGAATTCGGGGGGGGGCGG730.146No Hit
ATCGGGAGAGGGGCGGGGGGGGGAAGAGGGGAGAATTCGGGGGGGGCCGG660.132No Hit
ATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGAGAATTCGGGGGGGGCCGG620.124No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)